Library: Presentation: David Back

Slide 6 of 22

Speaker notes: Must be cautious when interpreting IC50 and IC95 data, because the numeric values generated depend on a number of factors relating to their in-vitro calculation, as listed above.

Particularly important for extrapolation of in-vitro data to an in-vivo context is the amount of protein in the culture medium:

in-vitro studies are commonly conducted with 50% human serum.

It is vital to have comparative data testing all drugs in the same system, under the same conditions, in order to be able to make a realistic comparison of IC50 or IC95 values.